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| This technology embodies a simple, inexpensive method for long term preservation of readily transformable callus cultures, and lines that have already been transformed, but for logistical reasons cannot be immediately progressed to callus growth and plant regeneration. Callus lines can now be frozen at any stage of the process from initial generation of a line, maintenance of the pre-transformation or post-transformation, or for sub-culturing. This technology can be used on a variety of callus types, unlike other methods. This method can be applied to maize, sorghum, wheat, rice, and others.cryopreserving plant callus satisfies need preserve actively growing transformable transformed chemicals alter physiology cells cryopreserve line permit recovery increased number recovered events entire isolation product development efficient spreading evenly physical order manipulate fragile minimal damage maintenance media supplemented osmoticum viability preserved slowly removing water controlled conditions dry storage future reviving stored begin glass fiber disc paper fine wire mesh similar material mannitol sucrose glucose function maintaining desiccate tissue chamber presence saturated solution Ca(NOsub3)sub2 4Hsub2 (NHsub4)sub2 SOsub4 mechanical device capable altering ambient humidity noted evaporative dehydration desiccated maintained submersion liquid nitrogen temperature -80degree room depending long general the longer survive revive further thawed variety temperatures 4degree 37degree decrease Preferentially proline desiccating closed container either storing thawing |
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Interested? This is an abstract of a technology that is available for sale or license. yet2.com can introduce you confidentially to the owner of this technology.
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