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| The present invention relates to development of Bifidobacteria strains with acid, bile salt and oxygen tolerance and their culture method. Bifidobacterium spp. is one kind important microflora which inhabits the intestinal tract of humans and animals. Many studies have indicated Bifidobacterium spp. exerts some health-promoting effects on its host. However, after oral administration, Bifidobacterium are overcome by the stress of gastrointestinal environment such as gastric acid and bile salt. Moreover, Bifidobacterium spp. is more anaerobic than other lactic acid bacteria. Products of Bifidobacterium spp. is very difficult to process and maintain the survivability during storage under aerobic condition……. |
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