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| The gene encoding the Acidovorax facilis 72W nitrilase has been cloned and over-expressed in E. coli, producing a microbial biocatalyst. A library of nitrilase mutants has been prepared from the original Acidovorax facilis 72W nitrilase, where these nitrilase mutants have also been cloned and over-expressed in E. coli. This proprietary collection of microbial nitrilase biocatalysts may find utility in the production of agrichemical and pharmaceutical intermediates, as well as specialty and commodity chemicals.isolated characterized nitrilase enzyme (EC 3557) Acidovorax facilis72W |
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