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| A detailed linkage analysis of the Ehd1 region was performed with a large segregating population, essential for map-based cloning. As a result, the Ehd1 gene promoting rice heading (flowering) was successfully isolated. Rice heading time was also found to be altered upon introduction of this gene. Based on these facts, the newly isolated and identified Ehd1 gene is expected to be useful in promoting plant flowering. Therefore, by transforming a sense strand of Ehd1 gene into a plant cultivar in which the function of this gene has been lost, rice heading (flowering) can be promoted under short-day conditions. At the same time, by introducing the Ehd1 gene in the antisense direction into a plant cultivar in which the function of the Ehd1 gene is retained, expression of the endogenous Ehd1 gene can be suppressed to delay heading (flowering). Since this alteration can be expected to occur not only under short-day conditions but also under long-day or natural day-length conditions, the present invention is effective in controlling heading time (flowering time) under various cultivation conditions. Since the period of time required for transformation is extremely short compared to that required for gene transfer by crossing, flowering time can be modified without altering other characteristics. The use of the isolated Ehd1 gene to promote flowering is expected to be useful in simple alteration of the flowering time of plants such as rice, and to contribute to the breeding of plants adapted to different locations.ice heading time (flowering time) mainly photoperiod sensitivity depends day length factors (basal vegetative growth temperature sensitivity) Genetic analysis performed date time-associated genes Se1 locus (chromosome 6) E1 7) E2 (unknown) E3 3) Ef1 10) discovered mutations variations inherent rice cultivars Recently DNA markers genetic analyses advanced characteristics exhibits complex inheritance (mapping quantitative trait loci (QTLs)) (Yano Plant Physiol 127 1425-1429 2001) great number remain isolated identified |
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